Protein Turnover in Mammalian Cell Cultures

Oxidative stress on mammalian cell cultures during recombinant protein expression

When the cell is under stress arising from oxidation, heat, infection, toxic contamination or any other stressful condition, proteins may unfold and expose residues in their structure that under normal physiological conditions are hidden and shielded from chemical reactions. In this licentiate thesis the effects of general oxidative stress on the production of recombinant protein by mammalian c...

Biosafety of Mammalian Cell Cultures

Enzyme activity levels in mammalian cell cultures.

Improved methods for growing animal cells in culture have led to their wide use as a tool in a variety of disciplines in biology. Cultures from many different anatomical origins are available and purification of several of them has been effected by the method of single clone isolation (1). The exact nature of the cultured cells and their relationship to the organ of their origin is, however, un...

Metabolic flux rewiring in mammalian cell cultures.

Continuous cell lines (CCLs) engage in 'wasteful' glucose and glutamine metabolism that leads to accumulation of inhibitory byproducts, primarily lactate and ammonium. Advances in techniques for mapping intracellular carbon fluxes and profiling global changes in enzyme expression have led to a deeper understanding of the molecular drivers underlying these metabolic alterations. However, recent ...

Enzvme Activitv Levels in Mammalian Cell Cultures*

Improved methods for growing animal cells in culture have led to their wide use as a tool in a variety of disciplines in biology. Cultures from many different anatomical origins are available and purification of several of them has been effected by the method of single clone isolation (1). The exact nature of the cultured cells and their relationship to the organ of their origin is, however, un...